About 20 protesters chanted slogans and waved signs at the traffic on First Avenue in Jamestown late Friday afternoon.
Slogans of "Hands up" "Don't shoot" and calls to end racism and racist police officers were chanted to the passing traffic. The protesters also waved signs carrying similar messages.
The messages echoed the Black Lives Matter protests that have occurred around the country in the wake of the death of George Floyd in Minneapolis on May 25 while in police custody.
Scott Edinger, Jamestown chief of police, said the department was aware of the protests.
"They've been peaceful and haven't caused any issues so we're pretty much letting them be," he said. "It is constitutionally protected free speech and they are doing it the proper way."
Protests in the same area Wednesday night drew counter-protests that stood across the street. Police officers said that incident had evolved to a shouting match but no conflicts or arrests resulted.
The driver of one vehicle was cited for exhibition driving after revving the vehicle's engine and squealing the tires.
During previous protests in Fargo, officials of the Jamestown Police Department and Stutsman County Sheriff's Office had sent officers to Fargo to assist law enforcement there.
Some drivers on First Avenue honked horns but none stopped at the protest. As of 5:15 p.m., there was no obvious police presence or counter-protests at the scene.